HTS of gDNA and RNA from Ugandan BL tumors identifies the repertoire of Ig rearrangements and sequence variants in tumor cells. (A-B) Cumulative frequency plots of all unique IGH (A) or IGK/IGL (B) sequences identified by HTS of gDNA from the Ugandan BL cohort. Each segment in the bar plots represents a unique nucleotide sequence, and the color indicates the type of Ig rearrangement. Black lines separate the unique sequences, so highly polyclonal regions appear black in the figure. *Indicates that the tumor sample was EBV-negative; †indicates that the repertoire was classified as polyclonal. (C) Normalized c-MYC expression for each of the 18 Ugandan tumors on which RNAseq analysis was performed. RNAseq was not performed on sample 009-0210 due to poor quality RNA. (D) Genes with at least 1 single nucleotide variant (SNV) detected in RNAseq and predicted to be pathologic in each of the 18 Ugandan BL tumors. A red box indicates the presence of at least 1 SNV.