Upregulation of GSTA2 in RUNX1 moderately attenuated AML cells. (A) Venn diagram showing the common genes that are upregulated in the RUNX1 moderately inhibited MV4-11 cells (sh_Rx1_moderate_1) while tightly downregulated in the RUNX1 profoundly inhibited ones (sh_Rx1_profound_1 and 2). (B) List of 21 genes extracted in panel A. Fold change of each gene was calculated by dividing the signal intensity of each gene in RUNX1 moderately inhibited MV4-11 cells by that in MV4-11 cells transduced with control (sh_Luc.). (C) GSTA2 expressions were determined in MV4-11 cells transduced with lentivirus encoding shRNA targeting Luciferase (sh_Luc.) or shRNAs against RUNX1 (sh_Rx1_moderate 1 or sh_Rx1_profound 1). Cells were incubated with 3 μM of doxycycline for 48 h, then total RNA was prepared and analyzed by RT-PCR. Values are normalized to those of control vector-transduced cells (n = 3). (D) Immunoblot of GSTA2, RUNX1, and GAPDH in MV4-11 cells used in panel C. (E) GSTA2 expressions were analyzed in the AML patients from TCGA clinical datasets as in Figure 1A (RUNX1 high: n = 62; RUNX1 intermediate: n = 63; RUNX1 low: n = 62). Data are mean ± SEM values. *P < .05; **P < .01, by 2-tailed Student t test.