Fibrin deposition in microfluidic model of inflammatory thrombosis. Fibrin deposition was monitored via red fluorescent antifibrin mAb during perfusion of WB through TNF-α–activated endothelialized channels at shear stress of 5 dynes/cm2. Accumulation of fibrin varies with increasing (A) concentration (1 ng/mL vs 10 ng/mL, each for 6 hours) and (B) duration (4 hours vs 6 hours, each 1 ng/mL) of TNF-α preactivation. Left panels show representative fluorescent images at various time points. Middle panel shows MFI vs time, with mean ± standard error of the mean (SEM) shown for n = 2 channels for control (no TNF) groups and n = 3 channels for each experimental group. Right panel shows AUC analyses. *P < .05 vs control; **P < .05 vs each other condition; #, not significant (P = .14).
