Both domains of hTM/R6.5 are required to generate an antithrombotic effect. (A) Experimental design. All proteins were infused in equimolar concentrations (50 nM) during the TNF washout period, followed by a 5-minute wash to remove nonspecifically bound protein. Flow of media and WB was at shear stress of 5 dynes/cm². (B) Only hTM/R6.5 inhibited fibrin deposition, confirming that both the ICAM-1 binding and hTM domains must be present to produce an antithrombotic effect. Left panel shows MFI vs time, mean ± SEM, with n = 2 channels per condition. Right panel shows AUC analysis. *P < .05 vs all other conditions.