Comparative testing of antithrombotic agents. (A) Experimental design, showing distinct timing of treatment with hTM/R6.5 scFv, bound to ECs during the first 25 minutes of the TNF-α washout period, vs soluble agents, which were mixed into WB. (B-C) Both 100 nM shTM (B) and 5 U/mL hirudin (C) inhibited coagulation but were less effective than hTM/R6.5 (50 nM). Left panels show MFI vs time, ± SEM, with n = 2 channels for TNF-α only and n = 3 channels for each therapeutic. Middle panels show representative fluorescent images. Right panels show AUC analyses. *P < .05 vs TNF only; **P < .05 vs both other conditions; #, not significant (P = .17).