hTM/R6.5 has greater antithrombotic activity than shTM in combined model of endothelial cytokine activation and endotoxemia. (A) Experimental design, showing 6-hr stimulation of endothelialized channels with TNF-α and 90-minute preactivation of WB with LPS (50 ng/mL). In this case, hTM/R6.5 (50 nM) was infused during the TNF-α washout period and mixed into the WB. (B) The fusion protein provided superior antithrombotic effect. Left panels show MFI vs time, mean ± SEM, with n = 2 channels for TNF/LPS only and n = 3 channels for each therapeutic. Right panels show AUC analyses. *P < .05 vs TNF/LPS only; **P < .05 vs both other conditions.