Cells with high expression of WT but not signaling-defective CD123 have impaired engraftment in mice. A total of 3 × 106 CD45.1 e14.5 murine FL cells transduced to express EV (CD123-low) and overexpress CD123 WT or mutant (CD123-high-WT or CD123-high-APIA) were transplanted into lethally irradiated recipient CD45.2 mice and allowed to engraft for 6 weeks. Flow cytometric analysis (A) transduced donor cells in recipient mouse BM using GFP, (B) total donor cells in recipient mouse BM using antibody to CD45.1, and (C) percentage of cells in BM with high expression of CD123. Total donor cell content of recipient mice was analyzed in peripheral blood (D) and spleen (F) and GFP expression of transduced cells in the circulation (E) and spleen (G). Each symbol represents an individual mouse. Statistical analysis: #nonsignificant difference, *P < .05, **P < .01, ***P < .001.
