Role of Fc-Effector function in cell mobilization and normal hematopoiesis. (A) Cell mobilization into the PB was detected by flow cytometry, using hCD45 and hCD33 Ab’s following a single dose of PF-06747143 (10 mg/kg) in MV4-11 AML tumor-bearing mice. Data represent mean hCD45⁺ and hCD33⁺ staining in PB relative to baseline (time 0 hours) ± SEM. (B) Cynomolgus monkeys (n = 3 per group) received a single dose of m15-IgG1 or m15-IgG4, at 50 mg/kg, or vehicle (control). Mean absolute PB WBC numbers ± SEM are shown over time. (C) Cynomolgus monkeys (n = 2 per group) received 2 weekly doses of PF-06747143 (10 mg/kg) or vehicle, at 0 and 168 hours. Mean absolute PB WBC numbers ± SEM are shown over time. (D) Cynomolgus monkeys (n = 8-12 per group) received 2 weekly doses of PF-06747143 (10 mg/kg), at 0 and 168 hours. PB number of hematopoietic progenitor CD34⁺ cells ± SEM, relative to each individual baseline level, are shown over time.