Kinetics of hT-ALL#1 engraftment in thoracic and tail vertebrae BM. (A, left) Hematoxylin and eosin histology staining of thoracic or tail vertebrae from NSG mice. (A, right) Absolute number of murine CD45+ hematopoietic cells per thoracic or tail vertebra (n = 15 per site) and triglyceride content of thoracic or tail vertebra (n = 4 per site). (B) Flow cytometry plots of hT-ALL#1 engraftment in thoracic or tail vertebrae as a function of time. (C) Kinetic analysis of hT-ALL#1 engraftment in thoracic or tail BM after injection of 106 primary hT-ALL cells per mice. (Left) Percent of leukemic cells. (Right) Absolute leukemic cell numbers. Two mice (3 to 6 vertebrae per niche and per mice) were euthanized per time point until signs of illness were observed. hT-ALL infiltration was evaluated among Ter119− cells. Other kinetic analyses are provided in supplemental Figure 1B-C. Statistics are calculated according to nonparametric Mann-Whitney U test (*P < .05; ***P < .001).
