Antithrombotic effects of 12-HETrE are partly dependent on IP. (A) Representative images of platelet (anti-GPIb; green) and fibrin (antifibrin; red) accumulation at the indicated times after laser-induced injury of cremaster muscle arteriole in WT mice treated with vehicle (n = 3; 23 thrombi) or 12-HETrE (6 mg/kg; n = 3; 24 thrombi) or IP^−/− mice treated IV with vehicle control (n = 3; 21 thrombi) or 12-HETrE (6 mg/kg; n = 3; 24 thrombi). Thrombus growth was quantified in real time after laser-induced injury of the cremaster muscle arterioles of WT or IP^−/− mice by measuring the mean fluorescence intensity (MFI) of platelet and fibrin accumulation. Laser-induced injury and subsequent imaging were acquired using a 3I intravital microscopy system on a Zeiss Examiner at ×63 magnification. Two-way analysis of variance. Data represent mean ± standard deviation (SD). (B) The time to vessel occlusion after FeCl₃-induced injury of the carotid artery was measured in WT and IP^−/− mice treated with either control (DMSO and PEG300) or 12-HETrE. Two-tailed paired t test was performed; **P < .01. Data represent mean ± SD.