Thrombin generation and vascular permeability in Meox2Cre-Thbd-null mice. (A) Calibrated automated thrombinography of plasma from control mice (Thbd^loxP/+, orange, n = 4) and Thbd-deficient mice (Thbd^−/−; blue, n = 4). (B) Thbd-null mice displayed prolonged lag time and time to peak thrombin generation (ttPeak), reduced endogenous thrombin potential (ETP), and peak thrombin generation (Peak). (C-D) Evans blue extravasation assay showing increased vascular permeability in the lungs of Thbd-null mice compared with controls Thbd^loxP/loxP; n = 5 per group). (D) Data shown in panel C were generated by quantitative scanning of fluorescence (at 720 nm) of whole-organ lung tissue.