RUNX1-mediated DNA demethylation in mitomycin C–treated 293T cells. (A) 293T cell growth after mitomycin C (MMC) treatment. 293T cells were treated with the indicated concentrations of MMC, and then cell densities were measured after 1, 3, and 6 days. Data are presented as means ± standard deviation (SD) of 3 biological replicates. (B) qMSP analysis of 3 RUNX1-mediated DNA demethylation target regions (RUNX1 targets) and 2 randomly selected negative control regions (Random) in mock vector–overexpressing, RUNX1-overexpressing, MMC-treated mock vector–overexpressing, and MMC-treated RUNX1-overexpressing 293T cells, respectively (Mock, RUNX1, MMC-mock, and MMC-RUNX1). The vertical axis represents ΔCt (unmethylated-specific primer-methylated-specific primer). Error bars represent SD. Asterisks denote significant difference: *P < .05, **P < .01; N.S., not significant. The experiments were performed in 3 biological replicates.