RUNX1-binding motif overrepresentation analysis of differentially methylated regions during hematopoietic development. (A) Differentiation hierarchy of hematopoietic development. (B) Scatter plots of M-values between induced pluripotent stem cells (iPSCs) and CD34+ hematopoietic progenitor cells (HPCs), between HPCs and CD14+ monocytes (MONs), between HPCs and CD19+ B cells (BC), and between HPCs and CD3+ T cells (TCs). Dashed black lines represent ΔM borders of >2. Green, purple, and gray dots represent significantly methylated, demethylated, and insignificant probes, respectively. Numbers of methylated and demethylated CpGs in each comparison are shown in the upper left and lower right of each plot, respectively. (C) Distribution of RUNX1-binding motif enrichment scores at ±5000 bp from DMCs. (D) Histogram showing distribution of RUNX1 ChIP-seq peaks in HPCs around demethylated regions of iPSC-HPCs (pink bars) and randomly sampled same number of probes (light blue bars). The overlapped regions are shown in purple.