Figure 4.
Figure 4. miR-26a plays an essential role in hematopoietic reconstitution after BMT. BM cells (5 × 105) transduced with miR-26a TuD inhibitor (miR-26 TuD) or control (ctrl) were transplanted into lethally irradiated congenic recipients. (A) Data shown are percent survival of recipients after BMT. Data were pooled from 2 experiments involving a total of 7 mice per group. (B) BM cells (CD45.2) transduced with ctrl or miR-26a TuD inhibitor (miR-26 TuD) were harvested and mixed with equal number of recipient-type BM cells (CD45.1) before transplantation into lethally irradiated congenic recipients (CD45.1). Representative plots of recipient peripheral blood (PB) leukocytes for ctrl or miR-26a TuD cells at 8 weeks after BMT (left). Reconstitution ratio of ctrl or miR-26a TuD donor cells in the recipients’ PB at 4, 8, and 20 to 30 weeks after transplantation (right). Data (mean ± SD) were pooled from 2 experiments involving a total of 10 mice per group. (C) Reconstitution ratio of ctrl or miR-26a TuD donor cells in the recipients’ PB B220+, CD3+, and Mac-1+ populations at 4, 8, and 20 to 30 weeks after BMT. Data (mean ± SD) were pooled from 2 experiments involving a total of 10 mice per group. (D) Reconstitution ratio of ctrl or miR-26 TuD donor cells in the BM, spleen, and thymus of recipients at 20 to 30 weeks after transplantation. Data (mean ± SD) were pooled from 2 experiments involving a total of 10 mice per group. (E) Reconstitution ratio of ctrl or miR-26a TuD donor cells in the LSK and HSC populations of the recipients’ BM at 20 to 30 weeks after BMT. Data (mean ± SD) were pooled from 2 experiments involving a total of 10 mice per group. (F) The percentages of annexin V+ in donor-derived LSK and HSC populations 5 days after BMT. Data (mean ± SD) were pooled from 2 experiments involving a total of 6 mice per group. (G) Relative expression level of Bak1 messenger RNA in donor-derived LSK and HSC populations. Data (mean ± SD) were pooled from 2 experiments involving a total of 6 mice per group. *P < .05, **P < .01. Error bars indicate SD.

miR-26a plays an essential role in hematopoietic reconstitution after BMT. BM cells (5 × 105) transduced with miR-26a TuD inhibitor (miR-26 TuD) or control (ctrl) were transplanted into lethally irradiated congenic recipients. (A) Data shown are percent survival of recipients after BMT. Data were pooled from 2 experiments involving a total of 7 mice per group. (B) BM cells (CD45.2) transduced with ctrl or miR-26a TuD inhibitor (miR-26 TuD) were harvested and mixed with equal number of recipient-type BM cells (CD45.1) before transplantation into lethally irradiated congenic recipients (CD45.1). Representative plots of recipient peripheral blood (PB) leukocytes for ctrl or miR-26a TuD cells at 8 weeks after BMT (left). Reconstitution ratio of ctrl or miR-26a TuD donor cells in the recipients’ PB at 4, 8, and 20 to 30 weeks after transplantation (right). Data (mean ± SD) were pooled from 2 experiments involving a total of 10 mice per group. (C) Reconstitution ratio of ctrl or miR-26a TuD donor cells in the recipients’ PB B220+, CD3+, and Mac-1+ populations at 4, 8, and 20 to 30 weeks after BMT. Data (mean ± SD) were pooled from 2 experiments involving a total of 10 mice per group. (D) Reconstitution ratio of ctrl or miR-26 TuD donor cells in the BM, spleen, and thymus of recipients at 20 to 30 weeks after transplantation. Data (mean ± SD) were pooled from 2 experiments involving a total of 10 mice per group. (E) Reconstitution ratio of ctrl or miR-26a TuD donor cells in the LSK and HSC populations of the recipients’ BM at 20 to 30 weeks after BMT. Data (mean ± SD) were pooled from 2 experiments involving a total of 10 mice per group. (F) The percentages of annexin V+ in donor-derived LSK and HSC populations 5 days after BMT. Data (mean ± SD) were pooled from 2 experiments involving a total of 6 mice per group. (G) Relative expression level of Bak1 messenger RNA in donor-derived LSK and HSC populations. Data (mean ± SD) were pooled from 2 experiments involving a total of 6 mice per group. *P < .05, **P < .01. Error bars indicate SD.

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