Bak1 is a critical target gene of miR-26a in hematopoietic reconstitution after BMT. BM cells (5 × 10⁵) transduced with miR-26a TuD inhibitor (miR-26 TuD) or control (ctrl) were transplanted into lethally irradiated congenic recipients. (A) Data shown are percent survival of recipients after BMT. Data were pooled from 2 experiments involving a total of 6 mice per group. (B) Bak1^+/+ or Bak1^−/− BM cells (CD45.2) transduced with ctrl or miR-26a TuD inhibitor (miR-26 TuD) were harvested and mixed with equal number of recipient-type BM cells (CD45.1) before transplantation into lethally irradiated congenic recipients (CD45.1). Representative plots of recipient peripheral blood (PB) leukocytes at 8 weeks after BMT (left). Reconstitution ratio of donor cells in the recipients’ PB at 4 and 8 weeks (wks) after transplantation (right). Data (mean ± SD) were pooled from 2 experiments involving a total of 6 mice per group. (C) Reconstitution ratio of donor cells in the recipients’ PB B220⁺, CD3⁺, and Mac-1⁺ populations at 4 and 8 wks after BMT. Data (mean ± SD) were pooled from 2 experiments involving a total of 6 mice per group. *P < .05, **P < .01. Error bars indicate SD.