Figure 1.
![Figure 1. Generation of FVIII-specific CD4 T cells from healthy individuals. CD4 T-cell lines were generated as described in Material and Methods. (A) FVIII-specific T-cell lines generated from donor #329. (B) Cross-reactivity analysis between KLH- and FVIII-specific T-cell lines. (C) Frequencies of CD4 T cells specific for FVIII and KLH estimated for 16 healthy blood donors. The frequency of CD4 T-cell precursors was calculated using Poisson distribution according to the following formula: Frequency = −Ln([number of negative wells/total number of wells tested])/(number of CD4 T cells per well). Statistical significance was assessed using the Wilcoxon match-pairs signed rank test. ****P < .0001. Ctrl, control.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/1/21/10.1182_bloodadvances.2017008706/3/advances008706f1.jpeg?Expires=1735823963&Signature=cSUKmGjfvYfUOROH-6mtrFE7ZLdCBV-feHw3luvFWLj4Ur5z4tfj75BNpkWQjsxLeCxsjcRHK~5-rHmpLhabz6Vt9QKrI-cnbPDT56Yl0Sc~GIrkCl30tJVjRTm-RAzeVQd6cIvWYkx5RSl2LNUFMEcjCib3ER0W83q80X-2lldVkDCc7GxRNvasDrABFjNj8j4fypdv7~iZ860t3cnnLo9z6qvhJwPDc~2ER7vXagzsqt1mN6S9EYObl~WZX2am90z2r8bT3lQwjOv36b3eYgynFOmJ5y98uBpA470VGilPDP7wCI4uZL7aA5PD8eCxvtUu-ldQ0~xCdDJ6ldaajQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Generation of FVIII-specific CD4 T cells from healthy individuals. CD4 T-cell lines were generated as described in Material and Methods. (A) FVIII-specific T-cell lines generated from donor #329. (B) Cross-reactivity analysis between KLH- and FVIII-specific T-cell lines. (C) Frequencies of CD4 T cells specific for FVIII and KLH estimated for 16 healthy blood donors. The frequency of CD4 T-cell precursors was calculated using Poisson distribution according to the following formula: Frequency = −Ln([number of negative wells/total number of wells tested])/(number of CD4 T cells per well). Statistical significance was assessed using the Wilcoxon match-pairs signed rank test. ****P < .0001. Ctrl, control.
