Figure 1.
Female NSG mice reconstituted with adult BM CD34+cells show higher B-cell chimerism than male recipients. Sublethally irradiated NSG and NSG-pct mice were injected with 1.5 × 105 to 2 × 105 BM CD34+ cells and grafted under the kidney capsule with a partially HLA-matched human fetal thymus. (A-D) Human peripheral reconstitution was measured by flow cytometry in PB every other week (10 HC donors; n = 58 mice). Means of multilineage chimerism among total (mouse plus human) PBMCs are shown over time. Bars represent standard error of the mean (SEM). (E-F) Mice were euthanized at week 3 after transplantation and BM (1 leg) was collected. Mean + SEM of human CD45 and CD19 percentage and number are shown. Each symbol represents a single mouse.
