B-cell levels in PB are higher in NSG and NS female vs male recipient mice injected with FL CD34+cells. Sublethally irradiated NSG or NS mice were injected with 1.5 × 105 to 2.5 × 105 FL CD34+ cells and grafted with autologous human fetal thymus under the kidney capsule. (A-D) Human peripheral chimerism was measured by flow cytometry in PB every 3 weeks (2 FL samples; n = 18 NSG and n = 12 NS mice). Means of multilineage chimerism among total (mouse plus human) PBMCs are shown over time. Bars represent + SEM. (E-F) Mice were euthanized between weeks 20 and 22 after transplantation, and BM and spleen were collected and analyzed. Mean + SEM of CD19 number are shown. Each square represents a single mouse. *P < .05 (statistically significant) calculated by Sidak’s multiple comparison test.
