Functional rescue strategies for Mpl mutants. Schematic summary of the 2 rescue approaches used to restore Mpl function: (1) overexpression of GRASP55 to force immature Mpl receptor expression at the cell surface using unconventional autophagy-dependent secretion and (2) CRISPR-Cas9 gene editing to convert mutated Mpl DNA sequence to WT sequence. sgRNA, single-guide RNA.