Flagellin-mediated LSK cell proliferation is TLR5 dependent. WT B6 mice, TLR5-knockout (shown as T5 KO), NLRC4-knockout (shown as N4 KO), and N4T5-DKO mice were treated with flagellin as in Figure 1. Whole bone marrow cells from these mice were analyzed for LSK cell proliferation by flow cytometry. Representative flow cytometry plots comparing LSK proliferation (A), LSK cell % in bone marrow (B), LSK cell number (C), BrdU incorporation (D) in WT vs N4T5-DKO mice, LSK cell percentage (E), and LSK cell number in bone marrow (F) in WT, TLR5-knockout, and NLRC4-knockout mice are shown. Statistics were analyzed by unpaired Student t test (*P < .05; **P < .01; ***P ≤ .001). Error bars represent SEM. ns, not significant.