TLR1/TLR2 activation induces apoptosis of AML cells via Caspase 3 activation. (A) In MA9 cells treated with Pam3CSK4, GSEA revealed enriched death pathway (3 hours; left panel) and apoptosis (24 hours; right panel) signatures. (B-E) MA9 cells treated with or without (control) Pam3CSK4 for 3 days. (B) Percentage of cleaved (active) Caspase 3⁺ cells and (C) percentage of Annexin V⁺ cells over time. (D) Cell count as determined by flow cytometry, using CountBright beads. Dotted line indicates amount of cells seeded. (E) Number of colonies formed in methylcellulose after 14 days. Shown in all graphs are data from 3 independent experiments, each with 3 replicates. Mean values and SDs are shown. ****P < .0001.