TLR1/TLR2 activation has mild effects on long-term bone marrow repopulating cells. 10 000 sorted LSK cells were cultured for 3 days with (CD45.1⁺CD45.2⁺, Pam3CSK4 group) or without (CD45.1⁺, control group) Pam3CSK4. Control and Pam3CSK4-treated cells were then pooled and transplanted into lethally irradiated CD45.2⁺ mice along with 200 000 support cells from CD45.2⁺ mice (12 mice per group). (A) Schematic picture of the experiment. (B) Percentage of donor cells in bone marrow and (C) lineage distribution within donor cells in the peripheral blood 16 weeks posttransplantation. Mean values and SDs are shown. ***P < .001.