Efficient correction of hematopoietic progenitors in total BM from FA-A patients with H/F-LVs encoding for FANCA. (A) Cytokine prestimulated hCD34⁺ cells were transduced with GFP encoding H/F-LVs at an MOI of 5 in 20% or 2% oxygen culture conditions. Three days after transduction, the percentage of GFP⁺ cells was determined by FACS. Data are representative of 3 independent experiments. (B) Cells from an unfractionated FA-A bone marrow sample (n = 3) were prestimulated for 18 hours and transduced with the GFP- or FANCA-encoding LVs as indicated in the presence of retronectin at an MOI of 5 and in low oxygen conditions (5%). CFC assays were performed 24 hours later in the absence or presence of mytomycin C (MMC).