Phenotype and functionality of MODP- and MOP-derived MΦs. Sorted MODPs and MOPs were plated at 3000 cells/well and cultured in the presence of M-CSF for 6 days. (A) Flow cytometric detection of indicated markers on MODP- and MOP-derived cells. (B) Flow cytometry plots showing the size of MODP- and MOP-derived MΦs, defined by F4/80⁺ phenotype (gated on DAPI⁻CD11b⁺ cells) according to forward scatter (FSC). (C) Frequency of small and big MΦs (CD11b⁺F4/80⁺ cells) in MODP- and MOP-derived cultures. (D-G) To evaluate phagocytosis capacity, we cocultured cells with FITC⁺ microbeads. (D) Microscopic illustration of cells containing green fluorescent beads in MODP- and MOP-derived MΦ cultures. (E) Flow cytometry plots depicting the percentage of FITC⁺F4/80⁺CD11b⁺ cells in MODP- and MOP-derived MΦ cultures. (F-G) Quantification by flow cytometry of FITC⁺F4/80⁺CD11b⁺ cells as indicated by number (#) and frequency (%). Data are representative of 3 experiments with n = 3. Error bars indicate standard error of the mean. **P < .01; ***P < .005; ****P < .0001.