Phenotype and functionality of MODP-derived DCs. (A-C) Sorted MODPs and MOPs were plated at 3000 cells/well and cultured in the presence of Flt3L and 1 ng/mL M-CSF for 8 days. (A) Microscopic illustration of MODP- and MOP-derived DC cultures at day 8. (B) FACS plots depicting the percentages of cDCs and pDCs within MHCII⁺CD11c⁺CD11b⁺ cells in MODP-derived DC cultures. (C) Detection of indicated markers on MODP-derived DCs after incubation with or without LPS for 16 h. (D-H) LPS-stimulated DCs were loaded with OVA or not and cocultured with CFSE-labeled, Vβ5⁺ CD8⁺ OT-I and CD4⁺ OT-II T cells. (D) Microscopic illustration of cocultures at day 3. (E) Quantification of T-cell expansion, expressed as absolute number (#) of live Vβ5⁺, CD8⁺, or CD4⁺ T cells per well (n = 2). (F-G) Histograms depicting the proliferation of CD8⁺ (F) and CD4⁺ (G) T-cell coculture, as detected by CFSE dilution. (H-I) Plots depicting CD69 expression on dividing CD8⁺ (H) and CD4⁺ (I) T cells. Data are representative of 3 experiments.