Transcriptome analysis of MODP and MOP. MODPs and MOPs were sorted from BM of 6 mice. Cell samples of 2 mice each were pooled to generate 3 biological replicates for RNAseq. Sequence reads were analyzed, as is indicated in the Materials and Methods section. (A) Hierarchical clustering and heat map of the 724 differentially expressed genes (P < .001; log2-fold change >4 or ≤4) from the 3 different samples (I, II, III). Upregulated and downregulated genes are ordered separately according to the degree of fold change expression (from “high” to “low”). The scale bar denotes log2 value. (B) GO network, generated by BiNGO. Nodes represent gene sets, and edges represent mutual overlap. Thus, highly redundant gene sets are grouped together as clusters, highlighted by the black circles. (C) GO analysis with IPA of the differentially expressed genes. Molecules with higher mRNA expression in the MOP are depicted in shades of red, whereas molecules with lower mRNA expression are in shades of green (log2 ratios). The molecules depicted clusters in the listed functional categories of genes, and their subcellular localization is also shown (extracellular space, plasma membrane, cytoplasm, and nucleus).