![Figure 1. Expression levels of Wnt5a-, ROR1-, and ROR2-signaling pathways in MCL cell lines and patient samples. (A) Immunoblot analysis (western blot [WB]) of ROR1- and ROR2-immunoprecipitated (IP) samples or of total cell lysates of MCL cell lines, CLL-like MEC-1 and MEC-2 cells (ROR1−), and Hela cells (ROR2+). (B) Percentage of ROR1+ cells in MCL patient samples used in this study, as described in Table 1. Error bar represents median with interquartile range. Relative ROR1 (C), Wnt5a (D), and ROR2 (E) mRNA expression levels in normal, MCL cell lines and patient samples, and CLL patient samples as derived from the National Center for Biotechnology Information Genomic Spatial Event (GSE) database, data collection number GSE86322 (for MCL cell lines) and the primary samples from GSE50006, GSE19243, GSE35426, GSE16455, GSE36000, GSE21452, and GSE70910. Comparison is done across sample within the same probe set. Error bars represent plus or minus standard deviation (SD).](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/1/24/10.1182_bloodadvances.2017010215/8/advances010215f1.jpeg?Expires=1735821784&Signature=g~2vOAXIX~gJALc-Gm5kGohZj9Q6vglZIEnXypUJRXBs9M-IMSR1yBjmP3fdFCYoRgfBGYvw8SlSUKbiIVzX9x7fCU2SYCVnU1iGa0kPFLJ-6BoIQwSxUq5KbRn-MSX9ltsFSiaPMNogYjxhvguaRFHMyOZ~MzwJBc2B6~rGqF~BIAk-GirYnkYDBb2ROns4Lya~YUD42RyaHsMOIYIYctqeU~g~tt~RVCck-FocwDkRWBmvrmANUwr6m7je8Lw0W8PV4Fus8dHehkc11NRq6QbLpF10SLJyVTcSARz3FKNbqHDvzkfDnxQDGwYOaboL5nkI066NFwr4FeIBSvyhvA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression levels of Wnt5a-, ROR1-, and ROR2-signaling pathways in MCL cell lines and patient samples. (A) Immunoblot analysis (western blot [WB]) of ROR1- and ROR2-immunoprecipitated (IP) samples or of total cell lysates of MCL cell lines, CLL-like MEC-1 and MEC-2 cells (ROR1−), and Hela cells (ROR2+). (B) Percentage of ROR1+ cells in MCL patient samples used in this study, as described in Table 1. Error bar represents median with interquartile range. Relative ROR1 (C), Wnt5a (D), and ROR2 (E) mRNA expression levels in normal, MCL cell lines and patient samples, and CLL patient samples as derived from the National Center for Biotechnology Information Genomic Spatial Event (GSE) database, data collection number GSE86322 (for MCL cell lines) and the primary samples from GSE50006, GSE19243, GSE35426, GSE16455, GSE36000, GSE21452, and GSE70910. Comparison is done across sample within the same probe set. Error bars represent plus or minus standard deviation (SD).
