Tagging does not influence the engraftment ability of HSC-enriched LSKSLAM cells. (A) Strategy of 10 LSKSLAM cell transplantation competition assay. Ten LSKSLAM cells from the Meis1^em1Bcca/Meis1^wt mice or the control mice (both are CD45.2) were transplanted together with 200 000 congenic BM cells (CD45.1) into lethally irradiated recipients (CD45.1). (B) Flow cytometric analysis from adult Meis1^em1Bcca/Meis1^wt BM indicating LSKSLAM cells have even higher GFP expression compared with the LSK population. (C) Percentages of donor-derived cells (CD45.2) in PB after 16 weeks of transplantation (P = .74; n = 10) suggest no significant difference in engraftment of Meis1^em1Bcca/Meis1^wt mouse BM cells vs WT mouse cells. (D) Flow cytometric analysis of the LSK population in recipient mouse BM at the time of euthanization (20 weeks after transplantation) demonstrates that the LSK cells from the Meis1^em1Bcca/Meis1^wt donor-derived cells remain GFP⁺.