Representative images of cells that were DNA transfected and immunostained. After fixation, cells were incubated with the anti-FORS1 monoclonal antibody and then with a biotinylated secondary antibody. After washing, they were treated with Vectastain ABC reagents. 3,3′-Diaminobenzidine was then used for peroxidase-mediated color development. Photos were taken of cells transfected with different expression constructs, as indicated at the top of the photos. The scale bar of 1 mm is indicated at the top right. del, deletion; Ex, exon.