![Figure 2. Effect of LMP1 expression upon TRAF3 availability to bind membrane CD40 or BAFFR in human BCL lines. (A-C) Western blots representative of 3 similar experiments. CD40 was immunoprecipitated from lysates of the indicated BCL-derived lines as described in “Methods.” Immunoprecipitates (IP) or whole-cell lysates (WCL) were subjected to SDS-PAGE and western blotting with antibodies specific for the indicated proteins. (D) DAUDI or SKW6.4 cells were untreated (left two lanes) or treated for 2 hours with 50 ng/mL recombinant human BAFF (rBAFF), after which BAFFR was immunoprecipitated from cell lysates. (E) Relative levels (mean ± standard error [SE]) of BAFFR-associated TRAF3 from 3 independent experiments. Statistical significance of differences was calculated as described in “Methods”; *P < .0008.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/1/27/10.1182_bloodadvances.2017009670/3/advances009670f2.jpeg?Expires=1735832066&Signature=AmfeYzht6bths24j8CydrpOw7mW8InEaQvP~PmUHceMtbzKtxGH0J1vd5kmpeJ2zFTj1dY4AnpyOxDlwVGuuTgIbdYeYbf68Xx5nY4U-JnIBp7RvC6XKRFLPVuWzN~fOinXIbwCUyqZuBI8RtIfDkCKvgFpYwUyyt9YKB101vS2QYLHFxG25Pwf7wjYO0dImYHUJsoLJ8xD7BGcCgE-ReYpD-zOTLJQxnREjYx84sPxq02zCied-qzzOjqH7GG1FNSOfjbCkJ12KjkrhXGq8HP3BZZfJoAcHlgTwg9emVoegwH~U87dVFw~XT7hM2ceBPeW5q9-fDwigYhGGsIAzIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of LMP1 expression upon TRAF3 availability to bind membrane CD40 or BAFFR in human BCL lines. (A-C) Western blots representative of 3 similar experiments. CD40 was immunoprecipitated from lysates of the indicated BCL-derived lines as described in “Methods.” Immunoprecipitates (IP) or whole-cell lysates (WCL) were subjected to SDS-PAGE and western blotting with antibodies specific for the indicated proteins. (D) DAUDI or SKW6.4 cells were untreated (left two lanes) or treated for 2 hours with 50 ng/mL recombinant human BAFF (rBAFF), after which BAFFR was immunoprecipitated from cell lysates. (E) Relative levels (mean ± standard error [SE]) of BAFFR-associated TRAF3 from 3 independent experiments. Statistical significance of differences was calculated as described in “Methods”; *P < .0008.
