Influence of LMP1 expression on responsiveness to IL-6 signaling. (A) The indicated cell lines were stimulated with recombinant mouse IL-6 (as described in “Methods”), and whole-cell lysates were subjected to SDS-PAGE and western blot analysis for the indicated proteins. A representative blot of 3 similar experiments is shown. (B) Relative STAT3 phosphorylation following IL-6 stimulation was quantified in 3 independent experiments, and mean values ± SE were calculated. A 2-way ANOVA was used to examine the results for statistical significance, and the adjusted P value was calculated using the Bonferroni method (n = 2 independent experiments; *P = .043, **P = .031).