Epinephrine/Gzsignaling is dispensable for platelet recruitment and activation. Laser-induced injury was performed in cremaster muscle arterioles of Gz+/+ (blue, green) and Gz−/− (red, magenta) mice with vehicle (circles) or the P2Y12 antagonist cangrelor (triangles, 0.75 µg prior to each injury). (A-B) CD41-positive area over time (A; mean ± SEM) and area under the CD41 vs time curve (AUC) (B; median and interquartile range). (C-D) The P-selectin positive area over time (C; mean ± SEM), and area under the P-selectin vs time curve (D; median and interquartile range). (E-F) Fibrin accumulation following laser injury was used as a measure of thrombin activity by quantifying the fibrin positive area over time (E; mean ± SEM). (F) Graph showing the area under the fibrin vs time curve. The line and error bars show the median and interquartile range. Gz+/+ plus vehicle, n = 36 injuries in 7 mice; Gz−/− plus vehicle, n = 43 injuries in 8 mice; Gz+/+ plus cangrelor, n = 52 injuries in 7 mice; Gz−/− plus cangrelor, n = 65 injuries in 8 mice.
