P2Y12signaling does not contribute to thrombin mediated platelet activation in the core region. Laser-induced injury was performed in cremaster muscle arterioles of wild-type mice treated with vehicle (blue), bivalirudin alone (0.5 µg/g, red), or bivalirudin plus cangrelor (0.75 µg prior to each injury, green) as described in “Materials and methods.” (A-B) Total platelet accumulation over time (A; mean ± SEM) and area under the CD41 vs time curve (AUC) (B; line and error bars show the median and interquartile range). (C-D) The P-selectin–positive area over time (C; mean ± SEM) and area under the P-selectin vs time curve (D; line and error bars show the median and interquartile range). (E-F) Fibrin accumulation over time (E; mean ± SEM) and the area under the fibrin vs time curve (F; median and interquartile range). Vehicle, n = 55 injuries in 12 mice; bivalirudin alone, n = 38 injuries in 6 mice; bivalirudin plus cangrelor, n = 29 injuries in 7 mice. The vehicle and bivalirudin alone groups shown are the same as those included in supplemental Figure 3.
