Single NKp44⁺ILC3s give rise to NK-like cells. Single-cell sorted tonsil-derived NKp44⁺ ILC3s were cultured on irradiated Cell Trace Violet–stained feeders in the presence of IL-2 plus IL-7 or IL-12 plus IL-15 and each well was individually assessed by flow cytometry after 3 weeks. (A) Representative flow cytometry plots of 2 clonal cultures. (B) Comparison between the frequency of the indicated populations in ILC3 clones and the corresponding bulk-sorted cultures. Data from 3 independent donors (mean in red ± SD, bars indicate median).