Chimerism and immune system reconstitution in Jinx mice after transfer of the human UNC13D gene into murine HSCs. (A) A schematic representation of the transplantation and infection protocol in mice. Sublethally irradiated (7 Gy) Jinx 45.1/2 recipients were reconstituted with Sca1⁺ murine HSCs transduced with a Munc13-4- or GFP-expressing LV from Jinx 45.2 mice (the J/Munc and J/GFP groups, respectively) or nontransduced Sca1⁺ cells from B6 45.1 mice (the J/B6 group). Each mouse received total number of 2.5 × 10⁵ Sca1⁺ cells. Mice 4 to 6 months posttransplantation were infected with LCMV (intraperitoneal injection, 200 pfu). Euthanasia time: 14 days after LCMV infection. (B) Flow cytometry analysis showing that more than 90% of the cells in the bone marrow and spleen were derived from the donor in all groups at euthanasia. (C) Flow cytometry analysis of various spleen cell subsets in the control groups (B6 and Jinx mice) and transplanted groups. Data are presented as the mean ± standard deviation percentage (first graph) and the absolute count (second graph) for each subset within the spleen. B6 (n = 10), Jinx (n = 10), J/GFP (n = 8), J/B6 (n = 11), and J/Munc (n = 12). *P < .05 using unpaired Student t test. (D) Flow cytometry analysis of Munc13-4/GFP (optimized human Munc13-4 fused to GFP) expression in Sca1⁺ cells, 48 hours after transduction (n = 3 mice).