Human Munc13-4 transgene integration and expression. (A) The transgene VCN and mRNA expression were measured by qPCR in the J/Munc group (absolute and relative amounts, respectively), and the result is presented as a correlation graph (r = 0.52; P = .04). (B) Flow cytometry analysis showing expression of the Munc13-4/GFP fusion protein 4 to 6 months after transplantation in the bone marrow (BM) for Sca1⁺ cells and in the spleen for various cell subsets.