Figure 1.
![Figure 1. Expression and function of the N411K PCFT mutant. (A) Influx of 0.5 μM [3H] MTX was assessed over 1 min at 37°C in RFC-/PCFT-null HeLa R1-11 cells transfected with wild-type PCFT, the N411K mutant, or vector control. Data are indicated as percentage of PCFT-WT activity and are the average of 3 independent experiments ± SEM. (B) Expression at the cell surface is depicted in the upper row. PCFT expression in the crude membrane preparation is depicted in the lower row. The western blot is representative of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/1/10.1182_bloodadvances.2017012690/3/advances012690f1.jpeg?Expires=1735609549&Signature=4IE7~nZH1Uws~ZRaqWjTYTS299~XsG35SsYyDtLSJSEt~HaEddZpWaX99VdGojqBQaciYg1e4lo0dl2V3S56tKc04WEGKLHtIlBRBX-clRQT67mlHlQBPc2rC5uLro0oQOmc67B1L83gdDpD6zAgPoqoZnMGnh9C1w54cyLhP-pWYnlapktc8hSWN~QDSmAxumLaarhonfNDHLJyNMlRJgKht-NKyfdKNnqtX~ZB155~sdVueoTsw2ZQeYdsptCO~di8nckDBKdjK4-K2pyj9OQJkB~qPs8Luwh1HpPXDKj23kcyGiK71frG90l1dnliChjLQPyi~eX-QTZVIMtWiw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression and function of the N411K PCFT mutant. (A) Influx of 0.5 μM [3H] MTX was assessed over 1 min at 37°C in RFC-/PCFT-null HeLa R1-11 cells transfected with wild-type PCFT, the N411K mutant, or vector control. Data are indicated as percentage of PCFT-WT activity and are the average of 3 independent experiments ± SEM. (B) Expression at the cell surface is depicted in the upper row. PCFT expression in the crude membrane preparation is depicted in the lower row. The western blot is representative of 3 independent experiments.
