Figure 2.
![Figure 2. Structural requirement at the Asn411 residue. (A) Expression at the cell surface (upper row), and in the crude membrane fraction (middle row), of the Asn411 mutants. β-Actin was the internal control (lower row). The blot is representative of 3 independent experiments. (B) Influx of 0.5 and 50 μM [3H] MTX was measured in HeLa R1-11 cells transfected with a variety of substitutions at the Asn411 residue. Data are the averages ± SEM of 3 independent experiments. *P < .01; **P < .0001, in comparison with PCFT-WT activity.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/1/10.1182_bloodadvances.2017012690/3/advances012690f2.jpeg?Expires=1735609714&Signature=z5XxT0IKU-28~t-E7kjYHlt~u2U6yj36S0k5fieJtCP0JzLyKM49wnUKRcWNUpcY-pGmZT7A75fb9DqIGHo8n6byzDOi50cdQPRTKOqd~SZ6KUoUi6WL6hNO0BfPVUaJX1h7WPVkI~lRxbbIwIQWW9eHt6I5Y9X3hmi-mJmReCluw5kFj2PiX6F3NLqI~JsQFaS9SoWKuSedAKaJA5Y51WBhAXJKSjBJHhCFXc-FsLop8jZgu7pv~GTasR2CIiCfFCvM4WmIERhDmcoyHwy5FzSCiogcFNvmKzzUeLG1KUvSiH5-ZJ1jAxX8vydajxGWlmQARI9mGYTzy6R2CoeLcQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Structural requirement at the Asn411 residue. (A) Expression at the cell surface (upper row), and in the crude membrane fraction (middle row), of the Asn411 mutants. β-Actin was the internal control (lower row). The blot is representative of 3 independent experiments. (B) Influx of 0.5 and 50 μM [3H] MTX was measured in HeLa R1-11 cells transfected with a variety of substitutions at the Asn411 residue. Data are the averages ± SEM of 3 independent experiments. *P < .01; **P < .0001, in comparison with PCFT-WT activity.
