Figure 3.
![Figure 3. Effects of sulfhydryl modifications of the N411C PCFT mutant. Cells harboring the N411C PCFT mutation were treated with 3 mM MTSES, 3 mM MTSET, or 0.5 mM MTSEA-biotin for 30 min at pH 7.4 and RT followed by assessment of [3H]MTX influx. Data are percentages of untreated cells ± SEM, from the average of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/1/10.1182_bloodadvances.2017012690/3/advances012690f3.jpeg?Expires=1735608839&Signature=AnRjyX2Oqz0B66VPkwMvubLmtjW1sc~XZFrW4Dk4Wi1iP3p~kO7lJMQy1vQWBGsHSSmvaQp6X4cWhQ1x1Tahx6Iz6LHxLC~rR1sg0t6LlOBFZ7pzGzH8GoT6mviZPCBCL1z6n8X9JrbjqWs8esxy5goe-oe7L4wi6pXV-drfO1r0Z0etbXVdKxRdYHn1nkHXV~Q0GQ8EVF7waOHUoH0vooPK7micyTRjREBSQhUEYJNdqEJEsrBqGYxppQIV3zrTgshMjSpR4Rjw6Prs4OMRH41BXDlEd3dNfDRjccjPZpWsVjm2dGkRuOrZHetPFgQADnEDmgVhbfQNeQhst~-Mpg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of sulfhydryl modifications of the N411C PCFT mutant. Cells harboring the N411C PCFT mutation were treated with 3 mM MTSES, 3 mM MTSET, or 0.5 mM MTSEA-biotin for 30 min at pH 7.4 and RT followed by assessment of [3H]MTX influx. Data are percentages of untreated cells ± SEM, from the average of 3 independent experiments.
