Figure 6.
![Figure 6. Pemetrexed influx kinetics. Influx of 0.05 to 5.0 µM [3H]pemetrexed was assessed in cells transfected with either PCFT-WT or the N411K mutant. Data are best fit to the Michaelis-Menten equation V = Vmax[S]/(Kt + [S]), where [S] is extracellular substrate concentration, Vmax is the maximum transport rate, and Kt is the concentration at which influx is one half of maximum. Data shown are the means ± SEM from 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/2/1/10.1182_bloodadvances.2017012690/3/advances012690f6.jpeg?Expires=1735608601&Signature=Jx95M6OcSyZP6vmufIofbHGdNsT68t5Hd~5WruXd--BWfVvgYAcz3zxtdsNBHenwAPALxjfaUv~aydjqO27DIEM4FjBukEft30oFJwTqEjzclKqPQk0omjDPHT30OBO50v3sauwN0ZXxMSfGsq2wjwS6RiXIY3SrzXLOMuygPS~WqI2QmK56tHnPjCjkkQfITRiX8iSoKAF28LM3-WBumQMtcJBWZ2ZurIUYnijtfWxux~gFjxcv6B2NZi9UgeMRoRUMpC7Hnd1op1yUuy2Sfmrs6VwsCEXQ7vxCeGWrJPSUvX4NFiKfi5cgVmLY8e-hmHQHEWqi~g6yUWHAFKumtA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pemetrexed influx kinetics. Influx of 0.05 to 5.0 µM [3H]pemetrexed was assessed in cells transfected with either PCFT-WT or the N411K mutant. Data are best fit to the Michaelis-Menten equation V = Vmax[S]/(Kt + [S]), where [S] is extracellular substrate concentration, Vmax is the maximum transport rate, and Kt is the concentration at which influx is one half of maximum. Data shown are the means ± SEM from 3 independent experiments.
