Purification of exosomes and MVs from tumor cells. Representative schema for the purification of exosomes and MVs by differential centrifugation. Tumor cells were seeded in dishes (1) and cultured for 5 days (2). The conditioned media were collected (3) and centrifuged at 500g (4) to eliminate the cells (5), and the supernatant was collected (6) and centrifuged at 2000g (7) to eliminate the cell debris (8). To isolate MVs, the supernatant was collected (9a) and centrifuged at 20 000g (10a). This pellet was used as a source of “MVs” (11a). To isolate the exosomes, the supernatant was collected (9b) and centrifuged at 10 000g (10b) to eliminate the large particles (11b). Then, the supernatant was collected (12b) and centrifuged at 100 000g (13b). The pellet was used as a source of “exosomes” (14b).