TLR2 ligand inhibits ferroportin expression. (A) THP-1 cells were stimulated with TLR2 or TLR4 ligand, and ferroportin expression was quantified by qRT-PCR. GAPDH expression was used as a control. (B) Surface ferroportin staining in THP-1 cells treated with TLR2 or TLR4 ligands was assessed by immunofluorescence microscopy (original magnification ×630). (C) MFI was assessed from 5 microscopic fields in panel B. (D) Surface ferroportin expression in THP-1 cells treated with TLR2 or TLR4 ligands compared with untreated cells was assessed by flow cytometry. All data were from 3 independent experiments. **P < .01, ***P < .001. DAPI, 4′,6-diamidino-2-phenylindole; FPN, ferroportin.