TLR-mediated hepcidin induction is independent of IL-6 signaling. (A-B) IL-8 expression in TLR-treated macrophages and organized to show hepcidin noninducers (A) and hepcidin inducers (B). (C-D) IL-6 expression in TLR-treated macrophages and organized as in panels A and B. (E-F) IL-6 secretion in TLR-treated macrophages and organized as in panels A and B. (G) IL-6 secretion in macrophages treated with Pam3CSK4 or LPS for 24 hours. (H) Hepcidin secretion in macrophages treated with Pam3CSK4 or LPS for 24 hours in presence of 1 μg/mL of IL-6 receptor–blocking antibody. All data were from 3 independent experiments. **P < .01, ***P < .001. LOD, limit of detection.