BCG induces iron sequestration through hepcidin induction and ferroportin downregulation. (A-C) Transcriptional changes of hepcidin (A), ferroportin (B), and ferritin (C) expression in BCG-infected THP-1 cells differentiated with 50 nM of PMA for 24 hours. (D) Hepcidin mRNA levels of BCG-infected WT and ΔMyD88 THP-1 cells. (E) Hepcidin secretion in BCG- or L monocytogenes (L mono)–infected THP-1 cells differentiated as in panel A. (F) Ferroportin expression in L mono–infected THP-1 cells. All data were from 3 independent experiments. ***P < .001.