Morphological difference between cultured LCs and SVECs. (A) Representative FACS profiles of CD31 immunostaining of LC (left) or SVEC (right) monolayers cultured in vitro. Profiles of isotype controls are at the bottom of each panel. (B) Representative bright field photomicrographs (original magnification ×100) of LCs (top, left) and SVECs (bottom, left). (Right) Magnified images of representative cells in the square in left panels. Scale bars, 200 μm. Arrows indicate spike-like membrane protrusions in LCs. Both cell types in the paired images were simultaneously sorted and cultured in parallel. The images were captured with cells no later after passage 2 and represent 8 independent experiments from 5 donors. (C) Fluorescent microscopic images of actin filaments detected by phalloidin probes (original magnification ×100) in LCs (top, left) and SVECs (bottom, left). (Right) Magnified images of representative cells in the square in the left panels. Both cell types were cultured on the same surface under unstimulated condition. Green, actin; blue, DAPI. Scale bars, 200 μm.