Characteristics of proliferation of LCs and SVECs. (A) The GEA values of selected genes encoding growth factor receptors and BCL2 (upper), and growth factors (lower), as described in Figure 2A. (B) RT-qPCR analysis of VEGF2 (VEGF C), KDR (VEGFR2), FGF18, and BCL2, as described in Figure 2H. (C) Representative FACS histogram of cell surface expression of VEGFR2 by LCs and SVECs. (D) Percentage of VEGFR2 positive LCs and SVECs. Data are shown as mean ± SD, n = 3 (triplicate analyses). **P = .002189 by paired Student t test. (E) Days required to cover the entire surface of a well in 96-well plate after the initial seeding of an equal number of sorted LCs and SVECs. The result represents an average of 4 independent experiments for each line of LCs or SVECs established from a total of 5 different spleen samples. ***P = .000582 by 2-sample Student t test. (F) Representative bright field photomicrographs (original magnification ×40) of LC (left) or SVEC (right) monolayers after their initial passage. Inserted squares show magnified images. Scale bars, 500 μm. Paired images of LC and SVEC cultures were captured in the same experiment performed in parallel, started with the same seeding number, and were sorted from the same spleen sample. Similar pairwise comparisons have been conducted in at least 3 independent experiments from 5 different spleen samples. IGF1R, insulin-like growth factor 1 receptor.