Figure 6.
Hematopoietic factor and adhesion molecule production by LCs and SVECs. (A) The GEA values (as described in Figure 2A) of genes encoding factors supporting hematopoiesis. (B) RT-qPCR analysis of IL15, IL34, KITLG (SCF), CXCL12, JAG1, and JAG2, as described in Figure 2H. (C) The GEA values of genes encoding adhesion molecules. (D) RT-qPCR analysis of VCAM1, ESAM, and CDH11 (OB-cadherin), as described in Figure 2H. (E) Representative FACS histogram of SCF cell surface expression by LCs and SVECS. (F) Representative FACS histogram of intracellular IL-33 in fixed LCs and SVECS. Gate M1: IL-33+ based on IgG. Gate M2: IL-33high. (G) The percentage of IL-33high cells (Gate M2) relative to total LCs and SVECs. Data are shown as mean ± SD, n = 3 (triplicate analyses). *P = .02271 by paired Student t test. (H) Representative FACS histograms of VCAM1. (I) Percentage of VCAM1+ cells in SVECs and LCs. Data are shown as mean ± SD, n ≥ 3. **P = .007786 by 2-sample Student t test.