Acute effects of TLR2 agonists on cytokine production and white blood cell counts in M fascicularis. (A-D) After a single subcutaneous injection of either Pam₂CSK₄ (red bars, 1-mg/kg dose) or GSK3277329 (blue bars, 15-mg/kg dose), circulating levels of G-CSF (A), MCP-1 (C), and IL-6 (D) and absolute neutrophil counts (B) were measured at either 6 or 24 hours after injection. Bars represent mean ± SEM (3 animals per group). Error bars are not shown for baseline IL-6 values, because the values shown are at the limit of quantification level. ANOVA analysis shows a significant effect of treatment on these graphed parameters (P < .05).