Effect of a TLR2 agonist or G-CSF on chemotherapy-induced neutropenia. Symbols indicate mean ± SEM values. Error bars are not shown if the error is smaller than the size of the symbol. (A-D) Neutrophil (A), lymphocyte (B), monocyte (C), and red blood cell (D) counts were measured in M fascicularis treated with vehicle, cyclophosphamide (CYP), or CYP plus GSK3277329 (3 mg/kg) or CYP plus G-CSF (10 µg/kg) (n = 3 per group). The normal range of neutrophil numbers between 1 and 10 × 109 cells/L is noted by dashed lines (A). GSK3277329 or G-CSF were dosed daily for 14 days as indicated by the linked arrows. The first dose received was on day 5, and the last dose on day 19. Significant differences vs the CYP group were determined by 2-way ANOVA followed by Dunnett’s multiple comparisons post-hoc test (*P < .05, **P < .01, ***P < .005).
