Human T_CMexpress tissue-homing addressins and are found in healthy human peripheral tissues. (A-B) Comparison of the expression of skin-homing (CLA) and gut-homing (α4β7) addressins in T_CM and T_EM by CyTOF analyses from a representative human donor. The gating strategy used to identify T_CM and T_EM among human peripheral blood CD45RO⁺ CD45RA⁻ FOXP3⁻ memory T cells is shown. (C-D) Aggregate data of skin and gut addressin expression by (C) CD4⁺ and (D) CD8⁺ T_CM (white bars) and T_EM (black bars). T cells that expressed neither CLA nor α4β7 were designated as unassigned. The mean and standard error of the mean (SEM) of 4 donors are shown. (E-G) T_CM and T_EM have comparable expression of skin- and gut-homing addressins. The mean and SEM of the total assignable tissue-tropic populations (total tropism, the sum of skin and gut-tropic populations) for (E) CD4⁺ and (F) CD8⁺ T cells are shown. The mean and SEM of 6 donors are shown. (H) T_CM are present in noninflamed human peripheral tissues. T cells were isolated from noninflamed human tissues and analyzed by flow cytometry. T_CM as a percentage of the total T-cell population are shown for (H) individual representative samples and (I) pooled data. The mean and SEM of 5 skin, 2 lung, 3 colon, and 6 cervix samples are shown.